Preparation and evaluation of bacterial nano-cellulose/chitosan composite tubes as potential small-diameter vascular grafts

doi:10.3969/j.issn.2095-4344.2017.34.010

Abstract

Abstract:

BACKGROUND: There are still no applicable vascular grafts for clinical replacement of the small-diameter blood vessels (< 6 mm).

OBJECTIVE: To explore the possibility of bacterial nano-cellulose (BNC)/chitosan (CH) composite tubes as small-diameter artificial blood vessels.

METHODS: BNC/CH tubes were fabricated by introducing chitosan (0.5%, 1%, 2%) into BNC tubes. Physicochemical properties of BNC/CH tubes were analyzed including macro-morphology, microstructure, density, water-holding capacity, burst pressure, mechanical properties, blood compatibility and cytocompatibility.

RESULTS AND CONCLUSION: The results indicated that, as compared with the BNC tubes, the water permeability of BNC/CH tubes reduced, the density and water-holding capacity increased, as well as the axial and radial mechanical performance was enhanced, but ductility and elasticity of BNC/CH tubes were weakened. Regarding blood compatibility, hemolysis ratios of BNC and BNC/CH met the requirements of medical grade composites, while the plasma recalcification time showed that anticoagulation of BNC/CH tubes was slightly weaker than that of the BNC tubes. Cell compatibility experiments showed that porcine hip artery endothelial cells stably proliferated on the inner surface of both BNC and BNC/CH tubes, but the proliferation was more obvious on the BNC surface than on the BNC/CH surface

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Cellulose, Chitosan, Blood Vessel Prosthesis, Tissue Engineering

CLC Number:

R318

Li Xue, Tang Jing-yu, Bao Lu-han, Chen Lin, Hong Feng.

Preparation and evaluation of bacterial nano-cellulose/chitosan composite tubes as potential small-diameter vascular grafts

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(34): 5467-5473.

Figures/Tables 7

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